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CRH/CRF Polyclonal antibody

CRH/CRF Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 26848-1-AP

Print datasheet

Synonyms

CRH, CRF, Corticotropin-releasing hormone, Corticotropin-releasing factor, Corticotropin releasing factor

验证数据展示

  • WB analysis using 26848-1-AP

    WB analysis using 26848-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26848-1-AP (CRH/CRF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 26848-1-AP

    IHC staining of human placenta using 26848-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26848-1-AP (CRH/CRF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 26848-1-AP

    IHC staining of human placenta using 26848-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26848-1-AP (CRH/CRF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 26848-1-AP

    IF Staining of rat brain using 26848-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CRH/CRF antibody (26848-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inrat brain tissue, mouse brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26848-1-AP targets CRH/CRF in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CRH/CRF fusion protein Ag25226 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CRH/CRF
Synonyms CRH, CRF, Corticotropin-releasing hormone, Corticotropin-releasing factor, Corticotropin releasing factor
Calculated Molecular Weight 21 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC011031
Gene Symbol CRH
Gene ID (NCBI) 1392
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06850
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRH, also called CRF or corticoliberin, is a peptide hormone and neurotransmitter involved in the stress response. Marked reduction in this protein has been observed in association with Alzheimer disease. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery.

实验方案

Product Specific Protocols
WB protocol for CRH/CRF antibody 26848-1-APDownload protocol
IHC protocol for CRH/CRF antibody 26848-1-APDownload protocol
IF protocol for CRH/CRF antibody 26848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols