验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Planning an IHC experiment? We recommend our IHCeasy CNTROB Ready-To-Use IHC Kit. CNTROB primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 4 publications below |
产品信息
26880-1-AP targets CNTROB in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | CNTROB fusion protein Ag25454 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | centrobin, centrosomal BRCA2 interacting protein |
| 别名 | PP1221, LYST-interacting protein 8, LYST interacting protein 8, LIP8, Centrosomal BRCA2-interacting protein |
| 计算分子量 | 903 aa, 101 kDa |
| 观测分子量 | 60-70 kDa, 100-130 kDa |
| GenBank蛋白编号 | BC021134 |
| 基因名称 | CNTROB |
| Gene ID (NCBI) | 116840 |
| RRID | AB_2880667 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N137 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CNTROB antibody 26880-1-AP | Download protocol |
| IF protocol for CNTROB antibody 26880-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol The central scaffold protein CEP350 coordinates centriole length, stability, and maturation | ||
Elife Global cellular response to chemical perturbation of PLK4 activity and abnormal centrosome number. | ||
Mol Biol Cell The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis. | ||
