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CCNK Polyclonal antibody

CCNK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26933-1-AP

Print datasheet

Synonyms

CCNK, CPR4, cyclin K

验证数据展示

  • WB analysis using 26933-1-AP

    WB analysis using 26933-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26933-1-AP (CCNK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy CCNK Ready-To-Use IHC Kit. CCNK primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26933-1-AP targets CCNK in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen CCNK fusion protein Ag25556 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin K
Synonyms CCNK, CPR4, cyclin K
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC015935
Gene Symbol CCNK
Gene ID (NCBI) 8812
RRIDAB_2935464
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75909
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclin-K (CCNK) is also named as CPR4 and belongs to the cyclin family. CCNK is a regulatory subunit of cyclin-dependent kinases that mediates activation of target kinases (PMID:10574912). It plays a role in transcriptional regulation via its role in regulating the phosphorylation of the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A) (PMID:9632813). The Cyclin K/Cdk12 complex maintains genomic stability via regulation of expression of DNA damage response genes (PMID:22012619).

实验方案

Product Specific Protocols
WB protocol for CCNK antibody 26933-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols