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AP4S1 Polyclonal antibody

AP4S1 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 27202-1-AP

Print datasheet

Synonyms

AP 4 complex subunit sigma 1, AP47B, AP4S1, CLA20, CLAPS4, Sigma 1 subunit of AP 4, Sigma 4 adaptin, Sigma4 adaptin

验证数据展示

  • IF Staining of mouse brain using 27202-1-AP

    IF Staining of mouse brain using 27202-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using AP4S1 antibody (27202-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse brain using 27202-1-AP

    IHC staining of mouse brain using 27202-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27202-1-AP (AP4S1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 27202-1-AP

    IHC staining of mouse brain using 27202-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27202-1-AP (AP4S1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 27202-1-AP

    IF Staining of mouse brain using 27202-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using AP4S1 antibody (27202-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27202-1-AP targets AP4S1 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen AP4S1 fusion protein Ag25661 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adaptor-related protein complex 4, sigma 1 subunit
Synonyms AP 4 complex subunit sigma 1, AP47B, AP4S1, CLA20, CLAPS4, Sigma 1 subunit of AP 4, Sigma 4 adaptin, Sigma4 adaptin
Calculated Molecular Weight 17 kDa
Observed Molecular Weight17 kDa
GenBank Accession NumberBC001259
Gene Symbol AP4S1
Gene ID (NCBI) 11154
RRIDAB_3085936
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y587
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for AP4S1 antibody 27202-1-APDownload protocol
IF protocol for AP4S1 antibody 27202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols