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KIF20B Polyclonal antibody

KIF20B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27269-1-AP

Print datasheet

Synonyms

Cancer/testis antigen 90, CT90, KIF20B, kinesin family member 20B, Kinesin like protein KIF20B, KRMP1, M phase phosphoprotein 1, MPHOSPH1, MPP 1, MPP1

验证数据展示

  • IHC staining of human colon cancer using 27269-1-AP

    IHC staining of human colon cancer using 27269-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27269-1-AP (KIF20B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 27269-1-AP

    IHC staining of human colon cancer using 27269-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27269-1-AP (KIF20B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 27269-1-AP

    WB analysis using 27269-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27269-1-AP (KIF20B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHT-29 cells, mouse brain tissue, HepG2 cells, Jurkat cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27269-1-AP targets KIF20B in WB, IHC, ELISA applications and shows reactivity with human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, Mouse
Immunogen KIF20B fusion protein Ag26191 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin family member 20B
Synonyms Cancer/testis antigen 90, CT90, KIF20B, kinesin family member 20B, Kinesin like protein KIF20B, KRMP1, M phase phosphoprotein 1, MPHOSPH1, MPP 1, MPP1
Calculated Molecular Weight 211 kDa
Observed Molecular Weight214 kDa
GenBank Accession NumberNM_001284259
Gene Symbol KIF20B
Gene ID (NCBI) 9585
RRIDAB_2918121
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KIF20B antibody 27269-1-APDownload protocol
IHC protocol for KIF20B antibody 27269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols