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验证数据展示

  • IHC staining of human colon cancer using 27269-1-AP

    IHC staining of human colon cancer using 27269-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27269-1-AP (KIF20B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 27269-1-AP

    IHC staining of human colon cancer using 27269-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27269-1-AP (KIF20B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 27269-1-AP

    WB analysis using 27269-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27269-1-AP (KIF20B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

KIF20B Polyclonal antibody

KIF20B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27269-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, Mouse

应用

WB, IHC, ELISA

别名

Cancer/testis antigen 90, CT90, KIF20B, kinesin family member 20B, Kinesin like protein KIF20B, KRMP1, M phase phosphoprotein 1, MPHOSPH1, MPP 1, MPP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-29 cells, mouse brain tissue, HepG2 cells, Jurkat cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27269-1-AP targets KIF20B in WB, IHC, ELISA applications and shows reactivity with human, Mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, Mouse
免疫原 KIF20B fusion protein Ag26191 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin family member 20B
别名 Cancer/testis antigen 90, CT90, KIF20B, kinesin family member 20B, Kinesin like protein KIF20B, KRMP1, M phase phosphoprotein 1, MPHOSPH1, MPP 1, MPP1
计算分子量 211 kDa
观测分子量214 kDa
GenBank蛋白编号NM_001284259
基因名称 KIF20B
Gene ID (NCBI) 9585
RRIDAB_2918121
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KIF20B antibody 27269-1-APDownload protocol
IHC protocol for KIF20B antibody 27269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols