验证数据展示
经过测试的应用
Positive WB detected in | mouse ovary tissue, mouse testis tissue, rat ovary tissue |
Positive IHC detected in | mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
27331-1-AP targets Inhibin alpha in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | Inhibin alpha fusion protein Ag25999 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | inhibin, alpha |
别名 | INHA, Inhibin-α, Inhibinα, Inhibin-A, Inhibin α |
计算分子量 | 40 kDa |
观测分子量 | 40 kDa |
GenBank蛋白编号 | BC006391 |
基因名称 | Inhibin alpha |
Gene ID (NCBI) | 3623 |
RRID | AB_3085946 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P05111 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Inhibin, a heterodimeric member of the TGF family, consists of an alpha (α) subunit (coded by INHA) and a beta (β) subunit, which combine to produce either inhibin A (αβA) or inhibin B (αβB). Inhibin complexes negatively regulate follicle stimulating hormone secretion from the pituitary gland. Inhibins have also been implicated in regulating numerous cellular processes including cell proliferation, apoptosis, immune response and hormone secretion.
实验方案
Product Specific Protocols | |
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WB protocol for Inhibin alpha antibody 27331-1-AP | Download protocol |
IHC protocol for Inhibin alpha antibody 27331-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |