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SULF1 Polyclonal antibody

SULF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27438-1-AP

Print datasheet

Synonyms

Extracellular sulfatase Sulf-2 secreted form, Extracellular sulfatase Sulf-1, Extracellular sulfatase Sulf 1, EC:3.1.6.14, EC:3.1.6.1

验证数据展示

  • WB analysis using 27438-1-AP

    WB analysis using 27438-1-AP

    HT-1080 cell lysates were subjected to SDS PAGE followed by western blot with 27438-1-AP (SULF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 27438-1-AP

    IHC staining of mouse skeletal muscle using 27438-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27438-1-AP (SULF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 27438-1-AP

    IHC staining of mouse skeletal muscle using 27438-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27438-1-AP (SULF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHT-1080 cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27438-1-AP targets SULF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SULF1 fusion protein Ag26758 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sulfatase 1
Synonyms Extracellular sulfatase Sulf-2 secreted form, Extracellular sulfatase Sulf-1, Extracellular sulfatase Sulf 1, EC:3.1.6.14, EC:3.1.6.1
Observed Molecular Weight101 kDa
GenBank Accession NumberBC068565
Gene Symbol SULF1
Gene ID (NCBI) 23213
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWU6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SULF1 antibody 27438-1-APDownload protocol
IHC protocol for SULF1 antibody 27438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols