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验证数据展示

  • WB analysis using 27441-1-AP

    WB analysis using 27441-1-AP

    PMA, LPS, and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 27441-1-AP (MIP 1 alpha/CCL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MIP-1 alpha/CCL3 Polyclonal antibody

MIP-1 alpha/CCL3 Polyclonal Antibody for WB, ELISA
Cat No. 27441-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

C C motif chemokine 3, CCL 3, CCL3, CCL-3, chemokine (C C motif) ligand 3, G0S19 1, MIP 1 alpha, MIP 1 alpha/CCL3, MIP 1A, MIP-1 alpha, MIP1A, MIP-1α

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27441-1-AP targets MIP-1 alpha/CCL3 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 MIP-1 alpha/CCL3 fusion protein Ag26764 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C-C motif) ligand 3
别名 C C motif chemokine 3, CCL 3, CCL3, CCL-3, chemokine (C C motif) ligand 3, G0S19 1, MIP 1 alpha, MIP 1 alpha/CCL3, MIP 1A, MIP-1 alpha, MIP1A, MIP-1α
计算分子量 92 aa, 10 kDa
观测分子量10 kDa
GenBank蛋白编号BC071834
基因名称 CCL3/MIP-1 alpha
Gene ID (NCBI) 6348
RRIDAB_3085961
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10147
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chemokine (C-C motif) ligand 3 (CCL3), also known as MIP-1α, belongs to the family of chemokines. CCL3 has been found in the central nervous system and its cognate receptors, CCR1 and CCR5, have been reported to be expressed by astrocytes, microglia and neurons. CCL3 and its receptors, CCR1 and CCR5, also contribute to the development of bone disease in multiple myeloma by supporting tumor growth and regulating osteoclast differentiation. CCL3 is also associated with the regulation of cell growth, angiogenesis, and metastasis of different tumors such as melanoma, renal cell carcinoma, and colorectal cancer. Moreover, CCL3 enhances cell migration and metastasis by up-regulating matrix metalloproteinase-2 (MMP)-2 expression in chondrosarcoma cells.

实验方案

Product Specific Protocols
WB protocol for MIP-1 alpha/CCL3 antibody 27441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols