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验证数据展示

  • IHC staining of human colon using 27794-1-AP

    IHC staining of human colon using 27794-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Periostin antibody included
  • IHC staining of human colon using 27794-1-AP

    IHC staining of human colon using 27794-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Periostin antibody included

Periostin Polyclonal antibody

Periostin Polyclonal Antibody for IHC, ELISA
Cat No. 27794-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

PDLPOSTN, Osteoblast-specific factor 2, Osteoblast specific factor 2, OSF-2, OSF2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman colon tissue, human colon cancer tissue, human breast cancer tissue, human liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Periostin Ready-To-Use IHC Kit. Periostin primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27794-1-AP targets Periostin in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原 Periostin fusion protein Ag27145 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 periostin, osteoblast specific factor
别名 PDLPOSTN, Osteoblast-specific factor 2, Osteoblast specific factor 2, OSF-2, OSF2
GenBank蛋白编号BC106710
基因名称 Periostin
Gene ID (NCBI) 10631
RRIDAB_2880975
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15063
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Periostin (POSTN, PN), originally named as osteoblast-specific factor 2 (OSF-2), is a 90-kDa secreted protein which is now classified as a matricellular protein. It is present in a wide variety of normal adult tissues and fetal tissues, and has a role in bone, tooth and heart development and function. Studies show that periostin is overexpressed in a broad range of human cancer types, including lung, ovary, breast and colon cancers. Recent evidence reveals that periostin is expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour, and it is required to allow cancer stem cell maintenance.

实验方案

Product Specific Protocols
IHC protocol for Periostin antibody 27794-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols