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验证数据展示

  • WB analysis using 27889-1-AP

    WB analysis using 27889-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27889-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 27889-1-AP

    WB analysis of HeLa using 27889-1-AP

    WB result of NAP1L4 antibody (27889-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAP1L4 transfected HeLa cells.

  • Featured Product
  • KD/KO验证

NAP1L4 Polyclonal antibody

NAP1L4 Polyclonal Antibody for WB, ELISA
Cat No. 27889-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, ELISA

别名

hNAP2, NAP 2, NAP1L4, NAP2, NAP2L, Nucleosome assembly protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HuH-7 cells, mouse heart tissue, mouse testis tissue, rat heart tissue, U2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27889-1-AP targets NAP1L4 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 NAP1L4 fusion protein Ag27469 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleosome assembly protein 1-like 4
别名 hNAP2, NAP 2, NAP1L4, NAP2, NAP2L, Nucleosome assembly protein 2
计算分子量 375 aa, 43 kDa
观测分子量 52 kDa
GenBank蛋白编号BC022090
基因名称 NAP1L4
Gene ID (NCBI) 4676
RRIDAB_2881006
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99733
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAP1L4 is a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer.

实验方案

Product Specific Protocols
WB protocol for NAP1L4 antibody 27889-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols