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验证数据展示

  • WB analysis using 28321-1-AP

    WB analysis using 28321-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28321-1-AP (LYVE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat liver using 28321-1-AP

    IHC staining of rat liver using 28321-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28321-1-AP (LYVE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat liver using 28321-1-AP

    IHC staining of rat liver using 28321-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28321-1-AP (LYVE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LYVE1 Polyclonal antibody

LYVE1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28321-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

HAR, Extracellular link domain-containing protein 1, CRSBP-1, CRSBP1, CRSBP 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHUVEC cells, bEnd.3 cells, mouse heart tissue, rat heart tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inrat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

28321-1-AP targets LYVE1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 LYVE1 fusion protein Ag28131 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lymphatic vessel endothelial hyaluronan receptor 1
别名 HAR, Extracellular link domain-containing protein 1, CRSBP-1, CRSBP1, CRSBP 1
计算分子量 322 aa, 35 kDa
观测分子量70 kDa
GenBank蛋白编号BC026231
基因名称 LYVE1
Gene ID (NCBI) 10894
RRIDAB_3086043
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5Y7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LYVE1 is a 322-amino acid type-I integral membrane glycoprotein primarily expressed on lymphatic endothelial cells. LYVE1 is a CD44 homologue. It acts as a receptor and binds to both soluble and immobilized hyaluronan. LYVE1 may function in lymphatic hyaluronan transport and have a role in tumor metastasis.

实验方案

Product Specific Protocols
WB protocol for LYVE1 antibody 28321-1-APDownload protocol
IHC protocol for LYVE1 antibody 28321-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Exp Eye Res

Distinct Activation of M1 and M2 Macrophages in the Primary Pterygium Lymphangiogenesis

Authors - Soo Jin Lee
View Article