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LYVE1 Polyclonal antibody

LYVE1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 28321-1-AP

Print datasheet

Synonyms

HAR, Extracellular link domain-containing protein 1, CRSBP-1, CRSBP1, CRSBP 1

验证数据展示

  • WB analysis using 28321-1-AP

    WB analysis using 28321-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28321-1-AP (LYVE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat liver using 28321-1-AP

    IHC staining of rat liver using 28321-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28321-1-AP (LYVE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat liver using 28321-1-AP

    IHC staining of rat liver using 28321-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28321-1-AP (LYVE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHUVEC cells, bEnd.3 cells, mouse heart tissue, rat heart tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inrat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

28321-1-AP targets LYVE1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen LYVE1 fusion protein Ag28131 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lymphatic vessel endothelial hyaluronan receptor 1
Synonyms HAR, Extracellular link domain-containing protein 1, CRSBP-1, CRSBP1, CRSBP 1
Calculated Molecular Weight 322 aa, 35 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC026231
Gene Symbol LYVE1
Gene ID (NCBI) 10894
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5Y7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LYVE1 is a 322-amino acid type-I integral membrane glycoprotein primarily expressed on lymphatic endothelial cells. LYVE1 is a CD44 homologue. It acts as a receptor and binds to both soluble and immobilized hyaluronan. LYVE1 may function in lymphatic hyaluronan transport and have a role in tumor metastasis.

实验方案

Product Specific Protocols
WB protocol for LYVE1 antibody 28321-1-APDownload protocol
IHC protocol for LYVE1 antibody 28321-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Exp Eye Res

Distinct Activation of M1 and M2 Macrophages in the Primary Pterygium Lymphangiogenesis

Authors - Soo Jin Lee
View Article