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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 28345-1-AP

    WB analysis using 28345-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28345-1-AP (GDF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

GDF1 Polyclonal antibody

GDF1 Polyclonal Antibody for WB, ELISA
Cat No. 28345-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

GDF 1, GDF1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected infetal human brain tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28345-1-AP targets GDF1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen GDF1 fusion protein Ag28831 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name growth differentiation factor 1
Synonyms GDF 1, GDF1
Calculated Molecular Weight 39 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberNM_001492
Gene Symbol GDF1
Gene ID (NCBI) 2657
RRIDAB_3086045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP27539
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GDF1 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. Studies in rodents suggest that this protein is involved in the establishment of left-right asymmetry in early embryogenesis and in neural development in later embryogenesis.

实验方案

Product Specific Protocols
WB protocol for GDF1 antibody 28345-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols