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验证数据展示

  • WB analysis using 28550-1-AP

    WB analysis using 28550-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28550-1-AP (GRIK5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 28550-1-AP

    IHC staining of mouse brain using 28550-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28550-1-AP (GRIK5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GRIK5 Polyclonal antibody

GRIK5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28550-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EAA2, Glutamate receptor KA 2, GRIK2, GRIK5, KA2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28550-1-AP targets GRIK5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen GRIK5 fusion protein Ag29363 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutamate receptor, ionotropic, kainate 5
Synonyms EAA2, Glutamate receptor KA 2, GRIK2, GRIK5, KA2
Calculated Molecular Weight 109 kDa
Observed Molecular Weight~110 kDa
GenBank Accession NumberNM_001301030
Gene Symbol GRIK5
Gene ID (NCBI) 2901
RRIDAB_3086064
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16478
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GRIK5 antibody 28550-1-APDownload protocol
IHC protocol for GRIK5 antibody 28550-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols