SMCR7/MID49 Polyclonal antibody

SMCR7/MID49 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (3)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28718-1-AP

Print datasheet

Synonyms

MID49, SMCR7



经过测试的应用

Positive WB detected inJurkat cells, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 5 publications below

产品信息

28718-1-AP targets SMCR7/MID49 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse, pig
Immunogen SMCR7/MID49 fusion protein Ag30119 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Smith-Magenis syndrome chromosome region, candidate 7
Synonyms MID49, SMCR7
Calculated Molecular Weight 454 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC014973
Gene Symbol MID49
Gene ID (NCBI) 125170
RRIDAB_2881198
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96C03
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMCR7, also named as MID49, is a 49 kDa mitochondrial outer membrane protein which regulates mitochondrial morphology. SMCR7 can directly recruit the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. SMCR7 has two isoforms.

实验方案

Product Specific Protocols
WB protocol for SMCR7/MID49 antibody 28718-1-APDownload protocol
IHC protocol for SMCR7/MID49 antibody 28718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Mol Med

Characterisation of cold-induced mitochondrial fission in porcine aortic endothelial cells.

Authors - Leonard Quiring
mouseWB

Cell Rep

A PKD-MFF signaling axis couples mitochondrial fission to mitotic progression.

Authors - Evanthia Pangou
humanWB

Nat Commun

Auranofin targets UBA1 and enhances UBA1 activity by facilitating ubiquitin trans-thioesterification to E2 ubiquitin-conjugating enzymes

Authors - Wenjing Yan
mouseWB

Int J Biol Sci

Mitochondrial Dysfunction by FADDosome Promotes Gastric Mucosal Injury in Portal Hypertensive Gastropathy

Authors - Yuelin Xiao
humanWB

Technol Cancer Res Treat

The Role of MIEF2 in Cisplatin Sensitivity in KIRP Patients: Insights from Four-gene Mitochondrial Fusion RNA Markers

Authors - Yusong Hou