验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, U-251 cells, THP-1 cells, U-87 MG cells |
| Positive IHC detected in | rat testis tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28828-1-AP targets ATAT1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | ATAT1 fusion protein Ag28757 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 6 open reading frame 134 |
| 别名 | C6orf134, Nbla00487, MEC17, FLJ13158, alpha-TAT1 |
| 计算分子量 | 47 kDa |
| 观测分子量 | 42-45 kDa |
| GenBank蛋白编号 | NM_001031722 |
| 基因名称 | ATAT1 |
| Gene ID (NCBI) | 79969 |
| RRID | AB_2881219 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5SQI0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C6orf134 is a reported alias name for the human gene ATAT1 coding alpha-tubulin N-acetyltransferase 1, and specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. May affect microtubule stability and regulate microtubule dynamics. May be involved in neuron development. Western blot shows a band between 36 kDa and 45 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATAT1 antibody 28828-1-AP | Download protocol |
| IHC protocol for ATAT1 antibody 28828-1-AP | Download protocol |
| IF protocol for ATAT1 antibody 28828-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience MFN2 coordinates mitochondria motility with α-tubulin acetylation and this regulation is disrupted in CMT2A | ||
Biochem Biophys Res Commun NWD1 influences the extension of neuronal axons by regulating microtubule stability | ||
Cancer Cell Int Suppression of microtubule acetylation mediates the anti-leukemic effect of CDK9 inhibition
| ||
Cell Death Dis TMEM232 is required for the formation of sperm flagellum and male fertility in mice | ||
MedComm (2020) Matrix stiffness-induced α-tubulin acetylation is required for skin fibrosis formation through activation of Yes-associated protein
| ||
Biochim Biophys Acta Mol Cell Res Eml1 promotes axonal growth by enhancing αTAT1-mediated microtubule acetylation |
