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验证数据展示

  • WB analysis using 28873-1-AP

    WB analysis using 28873-1-AP

    Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28873-1-AP (Phospho-GRB10 (Ser476) antibody) at dilution of 1:1000 incubated at 4℃ overnight.

Phospho-GRB10 (Ser476) Polyclonal antibody

Phospho-GRB10 (Ser476) Polyclonal Antibody for WB, ELISA
Cat No. 28873-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, ELISA

别名

Grb 10, GRB IR, GRB10, GRB10 adapter protein, GRBIR, IRBP, KIAA0207, MEG1, Phospho-GRB10 (Ser476), RSS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inλ phosphatase treated NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28873-1-AP targets Phospho-GRB10 (Ser476) in WB, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 growth factor receptor-bound protein 10
别名 Grb 10, GRB IR, GRB10, GRB10 adapter protein, GRBIR, IRBP, KIAA0207, MEG1, Phospho-GRB10 (Ser476), RSS
计算分子量536aa,61 kDa; 594aa,67 kDa
观测分子量70-80 kDa
GenBank蛋白编号BC024285
基因名称 GRB10
Gene ID (NCBI) 2887
RRIDAB_2918210
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13322
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Growth factor receptor-bound protein 10 (GRB10) is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including the INSR and INS-like growth factor (IGF1R) receptors. It may play a role in mediating INS-stimulated ubiquitination of INSR, leading to proteasomal degradation. It has been reported that GRB10 is a direct substrate of mechanistic/mammalian target of mTOR and can be phosphorylated at Ser150, Ser428 and Ser476 identified as MAPK-mediated phosphorylation sites. (PMID: 15952796, 24746805, 21659605)

实验方案

Product Specific Protocols
WB protocol for Phospho-GRB10 (Ser476) antibody 28873-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols