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验证数据展示

  • WB analysis using 28925-1-AP

    WB analysis using 28925-1-AP

    Non-treated HeLa and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 28925-1-AP (Phospo-PLA2G4A (Ser505) antibody) at dilution of 1:3000 incubated at 4℃ overnight.

  • IF Staining of HeLa using 28925-1-AP

    IF Staining of HeLa using 28925-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated HeLa cells using Phospho-PLA2G4A (Ser505) antibody (28925-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Phospho-PLA2G4A (Ser505) Polyclonal antibody

Phospho-PLA2G4A (Ser505) Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28925-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

cPLA2, cPLA2 alpha, Cytosolic phospholipase A2, Phospho-PLA2G4A (Ser505), PLA2G4, PLA2G4A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inλ phosphatase treated HeLa cells
Positive IF/ICC detected inλ phosphatase treated HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28925-1-AP targets Phospho-PLA2G4A (Ser505) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase A2, group IVA (cytosolic, calcium-dependent)
别名 cPLA2, cPLA2 alpha, Cytosolic phospholipase A2, Phospho-PLA2G4A (Ser505), PLA2G4, PLA2G4A
计算分子量 749 aa, 85 kDa
观测分子量100-110 kDa
GenBank蛋白编号BC114340
基因名称 PLA2G4A
Gene ID (NCBI) 5321
RRIDAB_2918213
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP47712
储存缓冲液 PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLA2G4A, also named as cPLA2 (cytosolic phospholipase A2), is a highly conserved and widely expressed enzyme which can promote lipid mediator production. PLA2G4A plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It has been reported that the activity and expression levels of PLA2G4A are upregulated in several neurodegenerative diseases. PLA2G4A can be activated at the phosphorylation site of ser505 by MAP kinase. (PMID: 25838312, 8381049, 31238788)

实验方案

Product Specific Protocols
WB protocol for Phospho-PLA2G4A (Ser505) antibody 28925-1-APDownload protocol
IF protocol for Phospho-PLA2G4A (Ser505) antibody 28925-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols