验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, λ phosphatase treated NIH/3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy MAP2K2 Ready-To-Use IHC Kit. MAP2K2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IF | See 1 publications below |
产品信息
28955-1-AP targets Phospho-MEK2 (Thr394) in WB, IF, ELISA applications and shows reactivity with Human, Mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human, Mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase kinase 2 |
| 别名 | ERK activator kinase 2, FLJ26075, MAP kinase kinase 2, MAP2K2, MAPK/ERK kinase 2, MAPKK 2, MAPKK2, MEK 2, MEK2, MKK2, Phospho-MAP2K2 (Thr394), PRKMK2 |
| 计算分子量 | 44 kDa |
| 观测分子量 | 44 kDa |
| GenBank蛋白编号 | BC018645 |
| 基因名称 | MEK2 |
| Gene ID (NCBI) | 5605 |
| RRID | AB_2881235 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P36507 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-MEK2 (Thr394) antibody 28955-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Immunol Defining blood-induced microglia functions in neurodegeneration through multiomic profiling | ||
Pharm Biol Myrtenol improves brain damage and promotes angiogenesis in rats with cerebral infarction by activating the ERK1/2 signalling pathway. | ||
Int J Biol Sci Dual role of CD73 as a signaling molecule and adenosine-generating enzyme in colorectal cancer progression and immune evasion | ||
Cell Mol Biol Lett A specific super-enhancer actuated by berberine regulates EGFR-mediated RAS-RAF1-MEK1/2-ERK1/2 pathway to induce nasopharyngeal carcinoma autophagy | ||
Mol Med Rep Downregulation of microRNA‑221‑3p promotes angiogenesis of lipoprotein(a)‑injured endothelial progenitor cells by targeting silent information regulator 1 to activate the RAF/MEK/ERK signaling pathway |