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验证数据展示

  • WB analysis using 29068-1-AP

    WB analysis using 29068-1-AP

    Non-treated and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 29068-1-AP (Phospho-TBC1D1 (Ser707) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody as the loading control.

Phospho-TBC1D1 (Ser707) Polyclonal antibody

Phospho-TBC1D1 (Ser707) Polyclonal Antibody for WB, ELISA
Cat No. 29068-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

KIAA1108, Phospho-TBC1D1 (Ser707), TBC, TBC1, TBC1 domain family member 1, TBC1D1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCalyculin A treated HEK-293T cells

This antibody is equivalent to Ser700 in mice.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29068-1-AP targets Phospho-TBC1D1 (Ser707) in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1
别名 KIAA1108, Phospho-TBC1D1 (Ser707), TBC, TBC1, TBC1 domain family member 1, TBC1D1
计算分子量 133 kDa
观测分子量150-160 kDa
GenBank蛋白编号BC050321
基因名称 TBC1D1
Gene ID (NCBI) 23216
RRIDAB_3085341
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86TI0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBC1D1 belong to the family of RabGAPs and its physiological roles in glucose homeostasis and its implication in human diseases have already been characterized in skeletal muscle and adipocytes in a large number of independent studies. TBC1D1 including twophospho-tyrosine-binding (PTB) domains, a calmodulin-binding domain(CBD) and the func-tional GAP domain responsible for catalyzing GTP hydrolysis. TBC1D1 is phosphorylated by AMPK at Ser660 and Thr237, and by AKT at Thr596. The RabGAPs TBC1D1 is important regulators of glucose homeostasis inskeletal muscle and adipose tissue. (PMID: 31627187, PMID: 18477703)

实验方案

Product Specific Protocols
WB protocol for Phospho-TBC1D1 (Ser707) antibody 29068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols