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验证数据展示

  • WB analysis using 29219-1-AP

    WB analysis using 29219-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29219-1-AP (CBLL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 29219-1-AP

    IHC staining of human lung cancer using 29219-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29219-1-AP (CBLL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CBLL1 antibody included

CBLL1 Polyclonal antibody

CBLL1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29219-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

HAKAI, EC:2.3.2.27, E3 ubiquitin-protein ligase Hakai, c-Cbl-like protein 1, Casitas B-lineage lymphoma-transforming sequence-like protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, NCI-H1299 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CBLL1 Ready-To-Use IHC Kit. CBLL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29219-1-AP targets CBLL1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 CBLL1 fusion protein Ag30900 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Cas-Br-M (murine) ecotropic retroviral transforming sequence-like 1
别名 HAKAI, EC:2.3.2.27, E3 ubiquitin-protein ligase Hakai, c-Cbl-like protein 1, Casitas B-lineage lymphoma-transforming sequence-like protein 1
计算分子量 491 aa, 55 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC027460
基因名称 CBLL1
Gene ID (NCBI) 79872
RRIDAB_2918251
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ75N03
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cbl proto‐oncogene E3 ubiquitin protein ligase‐like 1, RNF188, Hakai (CBLL1) is an evolutionarily conserved E3 ubiquitin ligase containing a RING‐finger domain. CBLL1 contains a typical RING‐finger, short pTyr‐binding domain, and proline‐rich domain. CBLL1 expression is upregulated in human colon and gastric cancer tissues, and CBLL1 has been reported to induce anchorage‐dependent cell growth. CBLL1 immunostaining was observed in both the nuclei and cytoplasm of cancer cells. (PMID: 31124298, PMID: 31646569, PMID: 21191016)

实验方案

Product Specific Protocols
WB protocol for CBLL1 antibody 29219-1-APDownload protocol
IHC protocol for CBLL1 antibody 29219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols