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NADK Polyclonal antibody

NADK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29290-1-AP

Print datasheet

Synonyms

dJ283E3.1, FLJ13052, FLJ37724, NAD kinase, NADK, Poly(P)/ATP NAD kinase

验证数据展示

  • WB analysis using 29290-1-AP

    WB analysis using 29290-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29290-1-AP (NADK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 29290-1-AP

    IHC staining of human stomach cancer using 29290-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29290-1-AP (NADK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 29290-1-AP

    IHC staining of human ovary cancer using 29290-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29290-1-AP (NADK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, Hela cells, Neuro-2a cells, NIH/3T3 cells, rat kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29290-1-AP targets NADK in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen NADK fusion protein Ag30982 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NAD kinase
Synonyms dJ283E3.1, FLJ13052, FLJ37724, NAD kinase, NADK, Poly(P)/ATP NAD kinase
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC001709
Gene Symbol NADK
Gene ID (NCBI) 65220
RRIDAB_2918272
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95544
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

NAD kinase (NADK) is the sole NADP+-biosynthetic enzyme that catalyzes phosphorylation of NAD+ to yield NADP+ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target (PMID:21526340). It belongs to the NAD kinase family. NADK has 3 isoforms with the molecular mass of 49, 63 and 46 kDa. The catalytically active human NADK is a homotetramer (PMID:11594753).

实验方案

Product Specific Protocols
WB protocol for NADK antibody 29290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols