验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse heart tissue, rat heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29365-1-AP targets PPP2R5A in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, Mouse, Rat |
| 免疫原 | PPP2R5A fusion protein Ag31072 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 2, regulatory subunit B', alpha isoform |
| 别名 | B56A, PP2A B subunit isoform B alpha, PPP2R5A, PR61A, PR61alpha |
| 计算分子量 | 486 aa, 56 kDa |
| 观测分子量 | 50-56 kDa |
| GenBank蛋白编号 | BC022474 |
| 基因名称 | PPP2R5A |
| Gene ID (NCBI) | 5525 |
| RRID | AB_3086125 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15172 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein phosphatase 2, regulatory subunit B (B56), alpha isoform (PPP2R5A) is one of the regulatory subunits of the protein phosphatase 2A (PP2A), which is a major member of protein serine/threonine phosphatases in cells. PPP2R5A can regulate the cellular location, substrate specification and protein phosphatase function of PP2A, through which PPP2R5A plays an important role in many cellular activities. It has been reported that PPP2R5A relates with many diseases including cancers by regulating many crucial signaling pathways involved in P53, Bcl-2, CDK, MAPK, JAK/STAT, c-Myc and β-Catenin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP2R5A antibody 29365-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |