验证数据展示
经过测试的应用
| Positive WB detected in | unboiled mouse lung tissue, unboiled rat lung tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CLDN5 Ready-To-Use IHC Kit. CLDN5 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29767-1-AP targets Claudin 5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Claudin 5 fusion protein Ag29044 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | claudin 5 |
| Synonyms | CLDN5, CLDN 5, Claudin-5, Claudin5, BEC1 |
| Calculated Molecular Weight | 218 aa, 23 kDa |
| Observed Molecular Weight | 22 kDa |
| GenBank Accession Number | BC032363 |
| Gene Symbol | CLDN5 |
| Gene ID (NCBI) | 7122 |
| RRID | AB_2935477 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00501 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 27 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Claudin 5 antibody 29767-1-AP | Download protocol |
| IHC protocol for Claudin 5 antibody 29767-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
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