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验证数据展示

  • WB analysis using 29798-1-AP

    WB analysis using 29798-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29798-1-AP (NOM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 29798-1-AP

    IHC staining of mouse cerebellum using 29798-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29798-1-AP (NOM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 29798-1-AP

    IHC staining of mouse cerebellum using 29798-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29798-1-AP (NOM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NOM1 Polyclonal antibody

NOM1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29798-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

C7orf3, FLJ16401, NOM1, SGD1, SGD1 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells, SW 1990 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29798-1-AP targets NOM1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 NOM1 fusion protein Ag31423 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleolar protein with MIF4G domain 1
别名 C7orf3, FLJ16401, NOM1, SGD1, SGD1 homolog
计算分子量96KD
观测分子量96~100 kDa
GenBank蛋白编号NM_138400
基因名称 NOM1
Gene ID (NCBI) 64434
RRIDAB_3086164
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5C9Z4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nucleolar protein NOM1, which contains an MIF4G domain and an MA3 domain, was first isolated from the bone marrow of children with acute myeloid leukemia. NOM1 is highly conserved in a variety of species, including in yeast and in humans.

实验方案

Product Specific Protocols
WB protocol for NOM1 antibody 29798-1-APDownload protocol
IHC protocol for NOM1 antibody 29798-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols