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验证数据展示

  • WB analysis using 29843-1-AP

    WB analysis using 29843-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29843-1-AP (RALGAPA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 29843-1-AP

    IHC staining of mouse testis using 29843-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29843-1-AP (C20orf74 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RALGAPA2 Polyclonal antibody

RALGAPA2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29843-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, IHC, ELISA

别名

250 kDa substrate of Akt, AS250, bA287B20.1, C20orf74, dJ1049G11, dJ1049G11.4, FLJ12819, KIAA1272, p220, RALGAPA2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HUVEC cells, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29843-1-AP targets RALGAPA2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 RALGAPA2 fusion protein Ag31460 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 20 open reading frame 74
别名 250 kDa substrate of Akt, AS250, bA287B20.1, C20orf74, dJ1049G11, dJ1049G11.4, FLJ12819, KIAA1272, p220, RALGAPA2
计算分子量211KD
观测分子量230 kDa
GenBank蛋白编号NM_020343
基因名称 RALGAPA2
Gene ID (NCBI) 57186
RRIDAB_2923613
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ2PPJ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RALGAPA2 antibody 29843-1-APDownload protocol
IHC protocol for RALGAPA2 antibody 29843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols