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Uromodulin Polyclonal antibody

Uromodulin Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 29975-1-AP

Print datasheet

Synonyms

ADMCKD2, FJHN, HNFJ, MCKD2, THGP, THP, UMOD, uromodulin

验证数据展示

  • WB analysis of human urine using 29975-1-AP

    WB analysis of human urine using 29975-1-AP

    human urine sample were subjected to SDS PAGE followed by western blot with 29975-1-AP (Uromodulin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 29975-1-AP

    IHC staining of human kidney using 29975-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29975-1-AP (Uromodulin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 29975-1-AP

    IHC staining of human kidney using 29975-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29975-1-AP (Uromodulin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse kidney using 29975-1-AP

    IF Staining of mouse kidney using 29975-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Uromodulin antibody (29975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse kidney using 29975-1-AP

    IF Staining of mouse kidney using 29975-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Uromodulin antibody (29975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman urine sample
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29975-1-AP targets Uromodulin in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen Uromodulin fusion protein Ag32343 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name uromodulin
Synonyms ADMCKD2, FJHN, HNFJ, MCKD2, THGP, THP, UMOD, uromodulin
Calculated Molecular Weight 640 aa, 70 kDa
Observed Molecular Weight80-90 kDa
GenBank Accession NumberBC035975
Gene Symbol Uromodulin
Gene ID (NCBI) 7369
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07911
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Uromodulin, also known as THP (Tamm-Horsfall glycoprotein), is the most abundant protein excreted in the urine under physiological conditions. Uromodulin is synthesized by the kidney and localizes in cells lining the ascending limb of Henle and distal convoluted tubule. Uromodulin has been linked to water/electrolyte balance and to kidney innate immunity. Also, studies in knockout mice demonstrated that it has a protective role against urinary tract infections and renal stone formation. Mutations in the gene encoding uromodulin lead to rare autosomal dominant diseases, collectively referred to as uromodulin-associated kidney diseases. (PMID: 21654721)

实验方案

Product Specific Protocols
WB protocol for Uromodulin antibody 29975-1-APDownload protocol
IHC protocol for Uromodulin antibody 29975-1-APDownload protocol
IF protocol for Uromodulin antibody 29975-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols