验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| RIP | See 1 publications below |
产品信息
29980-1-AP targets HNRNPR in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, RIP |
| 经测试反应性 | Human, mouse |
| 文献引用反应性 | human |
| 免疫原 | HNRNPR fusion protein Ag30339 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | heterogeneous nuclear ribonucleoprotein R |
| 别名 | FLJ25714, hnRNP R, HNRNPR, HNRPR |
| 计算分子量 | 71 kDa |
| 观测分子量 | 65-82 kDa |
| GenBank蛋白编号 | BC001449 |
| 基因名称 | HNRNPR |
| Gene ID (NCBI) | 10236 |
| RRID | AB_2923626 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43390 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HNRNPR belongs to Heterogeneous nuclear ribonucleoproteins (hnRNPs), which are among the most abundant proteins in the eukaryotic nucleus and they play important roles in processing of precursor mRNA. hnRNP proteins have a modular structure consisting of one or more RNA binding domains and at least another auxiliary domain, which likely mediates protein-protein interactions but may also contribute to nucleic acid binding. The calcualted molecular weight of HNRNPR is 71 kDa, but the migration behavior of the 82 kDa translation product was indistinguishable from that of HNRNPR. (PMID: 9421497)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HNRNPR antibody 29980-1-AP | Download protocol |
| IHC protocol for HNRNPR antibody 29980-1-AP | Download protocol |
| IF protocol for HNRNPR antibody 29980-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Med Genet HnRNPR strongly represses splicing of a critical exon associated with spinal muscular atrophy through binding to an exonic AU-rich element | ||
Biochim Biophys Acta Mol Basis Dis HNRNPA2B1 and HNRNPR stabilize ASCL1 in an m6A-dependent manner to promote neuroblastoma progression |