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验证数据展示

  • WB analysis using 29992-1-AP

    WB analysis using 29992-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29992-1-AP (Fibulin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 29992-1-AP

    IHC staining of human lung cancer using 29992-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29992-1-AP (Fibulin 5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 29992-1-AP

    IHC staining of human colon cancer using 29992-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29992-1-AP (Fibulin 5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 29992-1-AP

    IHC staining of mouse lung using 29992-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29992-1-AP (Fibulin 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 29992-1-AP

    IHC staining of mouse colon using 29992-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29992-1-AP (Fibulin 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Fibulin 5 Polyclonal antibody

Fibulin 5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29992-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

ARMD3, DANCE, EVEC, FBLN5, FIBL 5, fibulin 5, FLJ90059, UP50, Urine p50 protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, mouse kidney tissue, rat heart tissue
Positive IHC detected inhuman colon cancer tissue, mouse lung tissue, mouse colon tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29992-1-AP targets Fibulin 5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag30835

Product name: Recombinant human FBLN5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 30-254 aa of BC022280

Sequence: GFDLDRQSGQCLDIDECRTIPEACRGDMMCVNQNGGYLCIPRTNPVYRGPYSNPYSTPYSGPYPAAAPPLSAPNYPTISRPLICRFGYQMDESNQCVDVDECATDSHQCNPTQICINTEGGYTCSCTDGYWLLEGQCLDIDECRYGYCQQLCANVPGSYSCTCNPGFTLNEDGRSCQDVNECATENPCVQTCVNTYGSFICRCDPGYELEEDGVHCSDMDECSFS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fibulin 5
别名 ARMD3, DANCE, EVEC, FBLN5, FIBL 5, fibulin 5, FLJ90059, UP50, Urine p50 protein
计算分子量 448 aa, 50 kDa
观测分子量65-70 kDa
GenBank蛋白编号BC022280
基因名称 Fibulin 5
Gene ID (NCBI) 10516
RRIDAB_2935495
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UBX5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibulin-5, also known as EVEC and DANCE, is an extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies.

实验方案

Product Specific Protocols
IHC protocol for Fibulin 5 antibody 29992-1-APDownload protocol
WB protocol for Fibulin 5 antibody 29992-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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