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验证数据展示

  • WB analysis using 30022-1-AP

    WB analysis using 30022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30022-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 30022-1-AP

    IF Staining of U2OS using 30022-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using HIP14; ZDHHC17 antibody (30022-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 30022-1-AP

    WB analysis using 30022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30022-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

HIP14; ZDHHC17 Polyclonal antibody

HIP14; ZDHHC17 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30022-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

ZDHHC17, HIP 14, EC:2.3.1.225, EC:2.3.1.-, DHHC domain-containing cysteine-rich protein 17

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293T cells, Raji cells, U-251 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30022-1-AP targets HIP14; ZDHHC17 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HIP14; ZDHHC17 fusion protein Ag32654 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger, DHHC-type containing 17
别名 ZDHHC17, HIP 14, EC:2.3.1.225, EC:2.3.1.-, DHHC domain-containing cysteine-rich protein 17
计算分子量 73 kDa
观测分子量 70 kDa
GenBank蛋白编号BC050324
基因名称 ZDHHC17
Gene ID (NCBI) 23390
RRIDAB_2935500
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IUH5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZDHHC17 is a neuronal palmitoyl transferase. Post-translational modification by the lipid palmitate is crucial for the correct targeting and function of many proteins. Polyglutamine expansions of huntingtin protein are responsible for the Huntington neurological disorder. ZDHHC17 gene encodes palmitoyl transferase huntingtin interacting protein (HIP14) which regulates palmitoylation and distribution of hungtingtin. The latter is implicated in the formation of inclusion bodies and enhanced neuronal toxicity. HIP14 was also shown to control neurotransmitter release.

实验方案

Product Specific Protocols
WB protocol for HIP14; ZDHHC17 antibody 30022-1-APDownload protocol
IF protocol for HIP14; ZDHHC17 antibody 30022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols