验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse brain tissue, HeLa cells, A2780 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30068-1-AP targets Rad51D in WB, ELISA applications and shows reactivity with Human, Mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, Mouse |
| 免疫原 | Rad51D fusion protein Ag32303 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAD51-like 3 (S. cerevisiae) |
| 别名 | HsTRAD, R51H3, RAD51 homolog D, RAD51 like 3 (S. cerevisiae), RAD51 like protein 3, Rad51D, RAD51L3, Trad |
| 计算分子量 | 35 kDa |
| 观测分子量 | 30-40 kDa |
| GenBank蛋白编号 | BC014422 |
| 基因名称 | Rad51D |
| Gene ID (NCBI) | 5892 |
| RRID | AB_2935508 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75771 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAD51L3, also named RAD51D, R51H3 and TRAD, belongs to the recA family and RAD51 subfamily. It is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA, and specifically to nicks in duplex DNA. The Heterodimer of RAD51L3 is about 70 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Rad51D antibody 30068-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
