• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HeLa using 30133-1-AP

    WB analysis of HeLa using 30133-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 30133-1-AP (PLCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 30133-1-AP

    WB analysis of HeLa using 30133-1-AP

    WB result of PLCD4 antibody (30133-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLCD4 transfected HeLa cells.

  • IHC staining of mouse testis using 30133-1-AP

    IHC staining of mouse testis using 30133-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30133-1-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 30133-1-AP

    IHC staining of human pancreas cancer using 30133-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30133-1-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

PLCD4 Polyclonal antibody

PLCD4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30133-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, rat, mouse

应用

WB, IHC, ELISA

别名

hPLCD4, Phospholipase C delta 4, phospholipase C, delta 4, PLC delta 4, PLCD4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, rat testis tissue, human testis tissue
Positive IHC detected inmouse testis tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30133-1-AP targets PLCD4 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, rat, mouse
免疫原 PLCD4 fusion protein Ag32651 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase C, delta 4
别名 hPLCD4, Phospholipase C delta 4, phospholipase C, delta 4, PLC delta 4, PLCD4
计算分子量 85-90 kDa
观测分子量90 kDa
GenBank蛋白编号BC006355
基因名称 PLCD4
Gene ID (NCBI) 84812
RRIDAB_2935518
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BRC7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLCD4(1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4), also known as hPLCD4. It is highly expressed in skeletal muscle and kidney tissues. It may be an important enzyme for intracellular Ca²⁺ mobilization in the zona pellucida-induced acrosome reaction. PLCD4 cooperates with nuclear PKC to regulate liver regeneration. Overexpression of PLCD4 upregulates Erk signaling pathway and proliferation (PMID: 15140260).

实验方案

Product Specific Protocols
WB protocol for PLCD4 antibody 30133-1-APDownload protocol
IHC protocol for PLCD4 antibody 30133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols