• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 30146-1-AP

    WB analysis using 30146-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30146-1-AP (SEPT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 30146-1-AP

    IHC staining of mouse brain using 30146-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30146-1-AP (SEPT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse brain using 30146-1-AP

    IP experiment of mouse brain using 30146-1-AP

    IP result of anti-SEPT3 (IP:30146-1-AP, 4ug; Detection:30146-1-AP 1:2000) with mouse brain tissue lysate 1280 ug.

  • FC experiment of HEK-293 using 30146-1-AP

    FC experiment of HEK-293 using 30146-1-AP

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human SEPT3 (30146-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

SEPT3 Polyclonal antibody

SEPT3 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA
Cat No. 30146-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), IP, ELISA

别名

septin 3, SEP3, Neuronal-specific septin-3, Neuronal specific septin 3, bK250D10.3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30146-1-AP targets SEPT3 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SEPT3 fusion protein Ag32890 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 septin 3
别名 septin 3, SEP3, Neuronal-specific septin-3, Neuronal specific septin 3, bK250D10.3
计算分子量 358 aa, 41 kDa
观测分子量40 kDa
GenBank蛋白编号BC111779
基因名称 SEPT3
Gene ID (NCBI) 55964
RRIDAB_2935520
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UH03
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEPT3 is a developmentally regulated phosphoprotein. SEPT3 is a member of the septin GTPase family, which can form polymers and contribute to the cytoskeleton. SEPT3 is involved in neuronal autophagy. In the process of autophagy regulation, the level of SEPT3 will change according to the autophagy protein. SEPT3 is specifically expressed in the brain (PMID: 15485489; 35932293).

实验方案

Product Specific Protocols
WB protocol for SEPT3 antibody 30146-1-APDownload protocol
IHC protocol for SEPT3 antibody 30146-1-APDownload protocol
IP protocol for SEPT3 antibody 30146-1-APDownload protocol
FC protocol for SEPT3 antibody 30146-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols