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AHR Polyclonal antibody

AHR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30242-1-AP

Print datasheet

Synonyms

Class E basic helix-loop-helix protein 76, bHLHe76, Ah receptor

验证数据展示

  • WB analysis using 30242-1-AP

    WB analysis using 30242-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30242-1-AP (AHR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 30242-1-AP

    IHC staining of human ovary cancer using 30242-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30242-1-AP (AHR antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AHR antibody included
  • IHC staining of human ovary cancer using 30242-1-AP

    IHC staining of human ovary cancer using 30242-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30242-1-AP (AHR antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AHR antibody included

经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy AHR Ready-To-Use IHC Kit. AHR primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30242-1-AP targets AHR in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen AHR fusion protein Ag32335 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aryl hydrocarbon receptor
Synonyms Class E basic helix-loop-helix protein 76, bHLHe76, Ah receptor
Calculated Molecular Weight 848 aa, 96 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC070080
Gene Symbol AHR
Gene ID (NCBI) 196
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35869
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

实验方案

Product Specific Protocols
WB protocol for AHR antibody 30242-1-APDownload protocol
IHC protocol for AHR antibody 30242-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols