验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, SKOV-3 cells, U-87 MG cells |
| Positive IF/ICC detected in | SKOV-3 cells |
Planning an IHC experiment? We recommend our IHCeasy FGF2 Ready-To-Use IHC Kit. FGF2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
30333-1-AP targets FGF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human |
| 文献引用反应性 | mouse |
| 免疫原 | FGF2 fusion protein Ag28653 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fibroblast growth factor 2 (basic) |
| 别名 | FGF-2, fibroblast growth factor 2, FGFB, FGF basic, FGF B |
| 计算分子量 | 31 aa |
| 观测分子量 | 21 kDa，23 kDa |
| GenBank蛋白编号 | NM_002006 |
| 基因名称 | FGF2 |
| Gene ID (NCBI) | 2247 |
| RRID | AB_3669711 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P09038 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, belongs to a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in at least five different isoforms with distinct properties.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FGF2 antibody 30333-1-AP | Download protocol |
| IF protocol for FGF2 antibody 30333-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |