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Species Reactivity
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Species Reactivity
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验证数据展示

  • WB analysis using 30527-1-AP

    WB analysis using 30527-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30527-1-AP (PI16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of SKOV-3 using 30527-1-AP

    IF Staining of SKOV-3 using 30527-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PI16 antibody (30527-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

PI16 Polyclonal antibody

PI16 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30527-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, rat

应用

WB, IF/ICC, ELISA

别名

CRISP 9, CRISP9, DKFZp586B1817, MSMBBP, peptidase inhibitor 16, PI 16, PI16, PSP94 binding protein, PSPBP, UNQ289/PRO328

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, rat heart tissue, SKOV-3 cells
Positive IF/ICC detected inSKOV-3 cells
Planning an IHC experiment? We recommend our IHCeasy PI16 Ready-To-Use IHC Kit. PI16 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30527-1-AP targets PI16 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, rat
免疫原 PI16 fusion protein Ag32556 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidase inhibitor 16
别名 CRISP 9, CRISP9, DKFZp586B1817, MSMBBP, peptidase inhibitor 16, PI 16, PI16, PSP94 binding protein, PSPBP, UNQ289/PRO328
计算分子量 463 aa, 49 kDa
观测分子量 100 kDa, 49 kDa
GenBank蛋白编号BC022399
基因名称 PI16
Gene ID (NCBI) 221476
RRIDAB_3086350
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UXB8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PI16 antibody 30527-1-APDownload protocol
IF protocol for PI16 antibody 30527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols