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MFSD2 Polyclonal antibody

MFSD2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 30555-1-AP

Print datasheet

Synonyms

NLS1, MFSD2a, Major facilitator superfamily domain-containing protein 2A, HsMFSD2A, HMFN0656

验证数据展示

  • WB analysis using 30555-1-AP

    WB analysis using 30555-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30555-1-AP (MFSD2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 30555-1-AP

    IHC staining of mouse brain using 30555-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30555-1-AP (MFSD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 30555-1-AP

    IHC staining of mouse brain using 30555-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30555-1-AP (MFSD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 30555-1-AP

    IF Staining of mouse brain using 30555-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MFSD2 antibody (30555-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 30555-1-AP

    IF Staining of mouse brain using 30555-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MFSD2 antibody (30555-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

30555-1-AP targets MFSD2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen MFSD2 fusion protein Ag33064 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name major facilitator superfamily domain containing 2
Synonyms NLS1, MFSD2a, Major facilitator superfamily domain-containing protein 2A, HsMFSD2A, HMFN0656
Calculated Molecular Weight 543 aa, 60 kDa
Observed Molecular Weight55-60 kDa
GenBank Accession NumberBC092414
Gene Symbol MFSD2
Gene ID (NCBI) 84879
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NA29
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MFSD2 is a transmembrane protein and sodium-dependent lysophosphatidylcholine transporter. MFSD2 is involved in the establishment of the blood-brain barrier and is required for brain growth and function.

实验方案

Product Specific Protocols
WB protocol for MFSD2 antibody 30555-1-APDownload protocol
IHC protocol for MFSD2 antibody 30555-1-APDownload protocol
IF protocol for MFSD2 antibody 30555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Bioeng Transl Med

Sound conditioning strategy promoting paracellular permeability of the blood-labyrinth-barrier benefits inner ear drug delivery

Authors - Xueling Wang
View Article