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验证数据展示

  • WB analysis using 30599-1-AP

    WB analysis using 30599-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30599-1-AP (VCAN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human endometrial cancer using 30599-1-AP

    IHC staining of human endometrial cancer using 30599-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30599-1-AP (VCAN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human endometrial cancer using 30599-1-AP

    IHC staining of human endometrial cancer using 30599-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30599-1-AP (VCAN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 30599-1-AP

    IHC staining of human stomach cancer using 30599-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30599-1-AP (VCAN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 30599-1-AP

    IHC staining of human stomach cancer using 30599-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30599-1-AP (VCAN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NIH/3T3 using 30599-1-AP

    IF Staining of NIH/3T3 using 30599-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using VCAN antibody (30599-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of NIH/3T3 using 30599-1-AP

    IF Staining of NIH/3T3 using 30599-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using VCAN antibody (30599-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

VCAN Polyclonal antibody

VCAN Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30599-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PG M, Large fibroblast proteoglycan, Glial hyaluronate-binding protein, GHAP, ERVR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman stomach cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30599-1-AP targets VCAN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen VCAN fusion protein Ag30151 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name versican
Synonyms PG M, Large fibroblast proteoglycan, Glial hyaluronate-binding protein, GHAP, ERVR
Calculated Molecular Weight 373 kDa
Observed Molecular Weight250-300 kDa, 550 kDa
GenBank Accession NumberNM_004385
Gene Symbol VCAN
Gene ID (NCBI) 1462
RRIDAB_3669736
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13611
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Versican, a component of the extracellular matrix and a member of the lectican family of proteoglycans, is the largest chondroitin sulfate proteoglycan and has an important role in the development of the heart, musculoskeletal system and central nervous system (CNS). The structure of versican is characterized by an approximately 550-kDa core protein consisting of an amino-terminal (G1) hyaluronan binding domain, a carboxy-terminal (G3) domain, and two central chondroitin sulfate glycosaminoglycan attachment domains, the α-GAG and β-GAG domains. Versican has four different isoforms that differ in the central GAG attachment domain: V0 (contains both α and β GAG domains), V1 (contains only the β domain), V2 (contains only the α domain) and V3 (devoid of both GAG domains)(PMID: 26385570).

实验方案

Product Specific Protocols
WB protocol for VCAN antibody 30599-1-APDownload protocol
IHC protocol for VCAN antibody 30599-1-APDownload protocol
IF protocol for VCAN antibody 30599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols