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验证数据展示

  • WB analysis using 30952-1-AP

    WB analysis using 30952-1-AP

    MDA-MB-231 cells lysates were subjected to SDS PAGE followed by western blot with 30952-1-AP (IGFBP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

IGFBP4 Polyclonal antibody

IGFBP4 Polyclonal Antibody for WB, ELISA
Cat No. 30952-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

BP 4, HT29 IGFBP, IBP 4, IBP4, IGF binding protein 4, IGFBP 4, IGFBP4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMDA-MB-231 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30952-1-AP targets IGFBP4 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 insulin-like growth factor binding protein 4
别名 BP 4, HT29 IGFBP, IBP 4, IBP4, IGF binding protein 4, IGFBP 4, IGFBP4
计算分子量28 kDa
观测分子量 28-40 kDa
GenBank蛋白编号NM_001552.3
基因名称 IGFBP4
Gene ID (NCBI) 3487
RRIDAB_3669792
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP22692-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGFBP4, also named as IBP4, is a 258 amino acid protein, which contains one IGFBP N-terminal domain and one thyroglobulin type-1 domain. IGFBP4 is the smallest member of the insulin-like growth factor binding protein family (IGFBP). It is a hepatic protein that plays a role in modulating the activity and bioavailability of IGF-I. The expression of IGFBP4 was found to increase under conditions of hypoxia. IGFBP4 is highly expressed in adipocytes and osteoblasts and is inhibitory of IGFs in vitro. (PMID: 28938423, PMID: 34646401).

实验方案

Product Specific Protocols
WB protocol for IGFBP4 antibody 30952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols