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验证数据展示

  • WB analysis using 31061-1-AP

    WB analysis using 31061-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31061-1-AP (IFI35 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IFI35 Polyclonal antibody

IFI35 Polyclonal Antibody for WB, ELISA
Cat No. 31061-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

FLJ21753, Ifi 35, IFI35, IFP 35, IFP35, interferon induced protein 35

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, Jurkat cells, SW480 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31061-1-AP targets IFI35 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen IFI35 fusion protein Ag34653 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon-induced protein 35
Synonyms FLJ21753, Ifi 35, IFI35, IFP 35, IFP35, interferon induced protein 35
Calculated Molecular Weight 31 kDa
Observed Molecular Weight31-35 kDa
GenBank Accession NumberBC001356
Gene Symbol IFI35
Gene ID (NCBI) 3430
RRIDAB_3669837
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP80217
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFI35 (interferon induced protein 35), also known as IFP35. It is expected to be located in cytoplasm, nucleus and extracellular space. The protein is expressed in a wide range of cell types, including fibroblasts, macrophages, and epithelial cells. Involved in several processes, including macrophage activation involved in immune response; positive regulation of defense response; and regulation of signal transduction. And the calculated molecular weight of IFI35 is 31 kDa.

实验方案

Product Specific Protocols
WB protocol for IFI35 antibody 31061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols