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验证数据展示

  • WB analysis of MC38 using 31129-1-AP

    WB analysis of MC38 using 31129-1-AP

    WB result of FABP4 antibody (31129-1-AP; 1:400; room temperature for 1.5 hours) with wild-type and FABP4 knockout MC38 cells.

  • WB analysis using 31129-1-AP

    WB analysis using 31129-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31129-1-AP (FABP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

FABP4 Polyclonal antibody

FABP4 Polyclonal Antibody for WB, ELISA
Cat No. 31129-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

fatty acid binding protein 4, adipocyte, Fatty acid binding protein 4, ALBP, AFABP, Adipocyte-type fatty acid-binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, MC38 cells, rat heart tissue, rat skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy FABP4/AP2 Ready-To-Use IHC Kit. FABP4/AP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31129-1-AP targets FABP4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fatty acid binding protein 4, adipocyte
别名 fatty acid binding protein 4, adipocyte, Fatty acid binding protein 4, ALBP, AFABP, Adipocyte-type fatty acid-binding protein
计算分子量15 kDa
观测分子量15 kDa
GenBank蛋白编号NM_001442.3
基因名称 FABP4
Gene ID (NCBI) 2167
RRIDAB_3669865
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15090
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fatty acid binding protein (FABP) 4 is a member of the FABP family which abundantly expressed, fatty acid carrier proteins. FABPs are capable of binding a variety of hydrophobic molecules such as long-chain fatty acids and are important for their uptake and intracellular trafficking. It was first identified as an adipocyte-specific protein, important for the maintenance of lipid and glucose metabolism. It is also detected in macrophages, where it participates in regulating inflammation and cholesterol trafficking via NFκB and PPAR. In more recent studies, FABP4 has been found in a variety of endothelial cells, where it has been identified as a target of VEGF and a regulator of cell proliferation and possibly angiogenesis. Pathologically, FABP4 has been associated with the development of metabolic syndrome, diabetes and cancer and vulnerability of atherosclerotic plaques. FABP4 has been identified as a novel prognostic factor for both adverse cardiovascular events and breast cancer.

实验方案

Product Specific Protocols
WB protocol for FABP4 antibody 31129-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols