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验证数据展示

  • WB analysis using 31150-1-AP

    WB analysis using 31150-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31150-1-AP (TBL3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 31150-1-AP

    IF Staining of A431 using 31150-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TBL3 antibody (31150-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

TBL3 Polyclonal antibody

TBL3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31150-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IF/ICC, ELISA

别名

SAZD, TBL3, transducin (beta) like 3, Transducin beta like protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells, mouse testis tissue
Positive IF/ICC detected inA431 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31150-1-AP targets TBL3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 TBL3 fusion protein Ag34900 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transducin (beta)-like 3
别名 SAZD, TBL3, transducin (beta) like 3, Transducin beta like protein 3
计算分子量 89 kDa
观测分子量89 kDa
GenBank蛋白编号BC010231
基因名称 TBL3
Gene ID (NCBI) 10607
RRIDAB_3669872
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ12788
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transducin (beta)-like 3 (TBL3) is a protein of 808 amino acids with an apparent molecular weight of 89 kilodaltons.In mice, Tbl3 is a newly recognized nucleolar protein with regulatory roles in ribosome biogenesis (doi: 10.5315/wjh.v3.i3.93). In zebrafish, Tbl3 plays a tissue-specific role in regulating cell cycle rate during development(PMID: 22659140).

实验方案

Product Specific Protocols
WB protocol for TBL3 antibody 31150-1-APDownload protocol
IF protocol for TBL3 antibody 31150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols