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TBL3 Polyclonal antibody

TBL3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31150-1-AP

Print datasheet

Synonyms

SAZD, TBL3, transducin (beta) like 3, Transducin beta like protein 3

验证数据展示

  • WB analysis using 31150-1-AP

    WB analysis using 31150-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31150-1-AP (TBL3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 31150-1-AP

    IF Staining of A431 using 31150-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TBL3 antibody (31150-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells, mouse testis tissue
Positive IF/ICC detected inA431 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31150-1-AP targets TBL3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen TBL3 fusion protein Ag34900 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transducin (beta)-like 3
Synonyms SAZD, TBL3, transducin (beta) like 3, Transducin beta like protein 3
Calculated Molecular Weight 89 kDa
Observed Molecular Weight89 kDa
GenBank Accession NumberBC010231
Gene Symbol TBL3
Gene ID (NCBI) 10607
RRIDAB_3669872
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ12788
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transducin (beta)-like 3 (TBL3) is a protein of 808 amino acids with an apparent molecular weight of 89 kilodaltons.In mice, Tbl3 is a newly recognized nucleolar protein with regulatory roles in ribosome biogenesis (doi: 10.5315/wjh.v3.i3.93). In zebrafish, Tbl3 plays a tissue-specific role in regulating cell cycle rate during development(PMID: 22659140).

实验方案

Product Specific Protocols
WB protocol for TBL3 antibody 31150-1-APDownload protocol
IF protocol for TBL3 antibody 31150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols