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验证数据展示

  • WB analysis using 31183-1-AP

    WB analysis using 31183-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31183-1-AP (Cullin 5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 31183-1-AP

    WB analysis using 31183-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31183-1-AP (Cullin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 31183-1-AP

    IHC staining of mouse brain using 31183-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31183-1-AP (Cullin 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 31183-1-AP

    IHC staining of mouse brain using 31183-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31183-1-AP (Cullin 5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cullin 5 Polyclonal antibody

Cullin 5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31183-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CUL5, CUL 5, VACM1, VACM 1, Cullin-5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, MCF-7 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31183-1-AP targets Cullin 5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Cullin 5 fusion protein Ag34620 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cullin 5
别名 CUL5, CUL 5, VACM1, VACM 1, Cullin-5
计算分子量 91 kDa
观测分子量80 kDa, 90 kDa
GenBank蛋白编号BC063306
基因名称 Cullin 5
Gene ID (NCBI) 8065
RRIDAB_3669887
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ93034
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The cullin family proteins are scaffold proteins for the RING finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. In humans, there are eight members of cullins, including CUL1, 2, 3, 4A, 4B, 5, 7, and CUL9 with an evolutionarily conserved cullin homology domain at the C-terminus, responsible for their interaction with the RING protein, assembling 8 cullin-based RING dependent E3 ubiquitin ligases to target thousands of substrates for ubiquitination and degradation (PMID: 32334051). Cullin 5 is the key scaffolding protein of Cullin RING ligase-5 (CRL-5). Cullin 5 plays positive roles in regulating cell growth, proliferation and physiological and other processes in the human body (PMID: 32194910). Cullin 5 has been detected in the form of double bands in the results of Western blot, which might be caused by neddylation (PMID: 36449095; 31294695).

实验方案

Product Specific Protocols
WB protocol for Cullin 5 antibody 31183-1-APDownload protocol
IHC protocol for Cullin 5 antibody 31183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols