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验证数据展示

  • WB analysis using 31464-1-AP

    WB analysis using 31464-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31464-1-AP (E2F6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 31464-1-AP

    IF Staining of Jurkat using 31464-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using E2F6 antibody (31464-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

E2F6 Polyclonal antibody

E2F6 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31464-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

E2F-6, E2F 6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IF/ICC detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31464-1-AP targets E2F6 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 E2F6 fusion protein Ag35112 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 E2F transcription factor 6
别名 E2F-6, E2F 6
计算分子量 32 kDa
观测分子量35-40 kDa
GenBank蛋白编号BC008348
基因名称 E2F6
Gene ID (NCBI) 1876
RRIDAB_3669998
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO75461
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

E2F6 is a member of the E2F family of TFs that control cell proliferation and cell fate. Cancer patients with high E2F6 expression in tumors, such as glioblastoma, breast cancer, and hepatocellular carcinoma, have poor prognosis.

实验方案

Product Specific Protocols
WB protocol for E2F6 antibody 31464-1-APDownload protocol
IF protocol for E2F6 antibody 31464-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols