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验证数据展示

  • IHC staining of mouse brain using 31486-1-AP

    IHC staining of mouse brain using 31486-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31486-1-AP (SLC38A7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 31486-1-AP

    IHC staining of mouse brain using 31486-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31486-1-AP (SLC38A7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 31486-1-AP

    IF Staining of mouse brain using 31486-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SLC38A7 antibody (31486-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC38A7 Polyclonal antibody

SLC38A7 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 31486-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF-P, ELISA

别名

SNAT7, Solute carrier family 38 member 7, Sodium-coupled neutral amino acid transporter 7, FLJ12724, FLJ10815

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31486-1-AP targets SLC38A7 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 SLC38A7 fusion protein Ag35081 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 38, member 7
别名 SNAT7, Solute carrier family 38 member 7, Sodium-coupled neutral amino acid transporter 7, FLJ12724, FLJ10815
计算分子量 50 kDa
GenBank蛋白编号BC001961
基因名称 SLC38A7
Gene ID (NCBI) 55238
RRIDAB_3670003
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9NVC3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC38A7 also known as SNAT7, is a system N transporter with a substrate preference for L-glutamine(PMID: 21511949). SLC38A7 symporter that selectively transports sodium ions and amino acids, such as L-glutamine and L-asparagine from the lysosome into the cytoplasm and may participate in mTORC1 activation (PubMed:28416685, PubMed:35561222). colocalization of Slc38a7 with neuronal markers in excitatory and inhibitory neurons, but not in astrocytes or glial cells.

实验方案

Product Specific Protocols
IHC protocol for SLC38A7 antibody 31486-1-APDownload protocol
IF protocol for SLC38A7 antibody 31486-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols