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验证数据展示

  • WB analysis using 31541-1-AP

    WB analysis using 31541-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31541-1-AP (TANC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 31541-1-AP

    IF Staining of HeLa using 31541-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TANC2 antibody (31541-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TANC2 Polyclonal antibody

TANC2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31541-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

ROLSA, rols, KIAA1636, KIAA1148

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31541-1-AP targets TANC2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 TANC2 fusion protein Ag35427 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 2
别名 ROLSA, rols, KIAA1636, KIAA1148
计算分子量219kDa
观测分子量220 kDa
GenBank蛋白编号NM_001394998
基因名称 TANC2
Gene ID (NCBI) 26115
RRIDAB_3670030
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9HCD6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tetratricopeptide repeat, ankyrin repeat, and coiled-coil containing 2 (TANC2), also known as KIAA1148 and KIAA1636, belongs to the TANC family. TANC2 is a large 220 kDa protein consisting of 1990 amino acid residues. Named after its domain architecture, it comprises tetratricopeptide (TPR) and ankyrin repeats (ANK), a coiled-coil domain, and a C-terminal PDZ-interacting motif. TANC2 is widely expressed in the developing human (in excitatory neurons and radial glial cells) and rodent brain as well as in the adult brain as a scaffold in the postsynaptic density (PSD) of excitatory neurons. As such, it facilitates cell signaling downstream of cell surface glutamate receptors through multi-protein complex formation with other PSD proteins (e.g. PSD95, SHANK1), influencing dendritic spine formation and synaptic strength (PMID: 33976205, 34964047).

实验方案

Product Specific Protocols
WB protocol for TANC2 antibody 31541-1-APDownload protocol
IF protocol for TANC2 antibody 31541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols