验证数据展示
经过测试的应用
Positive WB detected in | mouse colon tissue, mouse small intestine tissue, rat colon tissue |
Positive IHC detected in | mouse colon tissue, rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | rat liver tissue, mouse colon tissue |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
32189-1-AP targets EPCAM/CD326 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.
Tested Applications | WB, IHC, IF-P, ELISA Application Description |
Tested Reactivity | mouse, rat |
Immunogen | Recombinant protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | epithelial cell adhesion molecule |
Synonyms | Epithelial glycoprotein 314, Epithelial cell adhesion molecule, Ep-CAM, Epcam, EGP314 |
Calculated Molecular Weight | 35 kDa |
Observed Molecular Weight | 40 kDa |
GenBank Accession Number | NM_008532.2 |
Gene Symbol | Epcam |
Gene ID (NCBI) | 17075 |
RRID | AB_3670218 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity Purification |
UNIPROT ID | Q99JW5 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)
实验方案
Product Specific Protocols | |
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WB protocol for EPCAM/CD326 antibody 32189-1-AP | Download protocol |
IHC protocol for EPCAM/CD326 antibody 32189-1-AP | Download protocol |
IF protocol for EPCAM/CD326 antibody 32189-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |