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验证数据展示

  • WB analysis using 32189-1-AP

    WB analysis using 32189-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32189-1-AP (EPCAM/CD326 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 32189-1-AP

    IHC staining of mouse colon using 32189-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 32189-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 32189-1-AP

    IHC staining of mouse colon using 32189-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 32189-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse colon using 32189-1-AP

    IF Staining of mouse colon using 32189-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using EPCAM/CD326 antibody (32189-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat liver using 32189-1-AP

    IF Staining of rat liver using 32189-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using EPCAM/CD326 antibody (32189-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EPCAM/CD326 Polyclonal antibody

EPCAM/CD326 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 32189-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Epithelial glycoprotein 314, Epithelial cell adhesion molecule, Ep-CAM, Epcam, EGP314

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse colon tissue, mouse small intestine tissue, rat colon tissue
Positive IHC detected inmouse colon tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat liver tissue, mouse colon tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32189-1-AP targets EPCAM/CD326 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name epithelial cell adhesion molecule
Synonyms Epithelial glycoprotein 314, Epithelial cell adhesion molecule, Ep-CAM, Epcam, EGP314
Calculated Molecular Weight35 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberNM_008532.2
Gene Symbol Epcam
Gene ID (NCBI) 17075
RRIDAB_3670218
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ99JW5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

实验方案

Product Specific Protocols
WB protocol for EPCAM/CD326 antibody 32189-1-APDownload protocol
IHC protocol for EPCAM/CD326 antibody 32189-1-APDownload protocol
IF protocol for EPCAM/CD326 antibody 32189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols