验证数据展示
经过测试的应用
| Positive WB detected in | multi-cells/tissue, HSkMC cells, human skeletal muscle tissue |
| Positive IHC detected in | human skeletal muscle tissue, human bladder tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55069-1-AP targets MYH2-specific in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human |
| Cited Reactivity | mouse, rat, pig, canine |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | myosin, heavy chain 2, skeletal muscle, adult |
| Synonyms | Myosin 2, MYH2, Myosin heavy chain 2, MYHSA2, MyHC-IIa |
| Calculated Molecular Weight | 223 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | NM_017534 |
| Gene Symbol | MYH2 |
| Gene ID (NCBI) | 4620 |
| RRID | AB_10860267 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UKX2 |
| Storage Buffer | 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYH2 (Myosin II) is a member of the class II or conventional myosin heavy chains. Myosin II was first isolated from muscle but is also found in non muscle cells, and it is especially enriched in highly motile cell types such as amoebae. It forms bipolar filaments that interact with actin filaments to produce contraction. And it is the motor protein that generates force to drive muscle contraction. Functions in skeletal muscle contraction. The antibody is specific to MYH2, will not bind other myosins. It is a skeletal muscle specific antibody.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYH2-specific antibody 55069-1-AP | Download protocol |
| IHC protocol for MYH2-specific antibody 55069-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Loss of Myostatin Alters Mitochondrial Oxidative Phosphorylation, TCA Cycle Activity, and ATP Production in Skeletal Muscle | ||
BMC Genomics Identification of different myofiber types in pigs muscles and construction of regulatory networks | ||
Animals (Basel) Comparative Analysis of Muscle Fibers in Selected Muscles of Working and Companion Dog Breeds | ||
Sci Rep Dietary supplementation with inulin improves burn-induced skeletal muscle atrophy by regulating gut microbiota disorders |
