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验证数据展示

  • WB analysis using 55361-1-AP

    WB analysis using 55361-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55361-1-AP (PRDM16 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 55361-1-AP

    IHC staining of human stomach using 55361-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55361-1-AP (PRDM16 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRDM16 antibody included

PRDM16 Polyclonal antibody

PRDM16 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55361-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

MEL1, MDS1/EVI1-like gene 1, MDS1/EVI1 like gene 1, KIAA1675, Histone-lysine N-methyltransferase PRDM16

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, NCI-H1299 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

PRDM16 has low expression in most cells and tissues

Planning an IHC experiment? We recommend our IHCeasy PRDM16 Ready-To-Use IHC Kit. PRDM16 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55361-1-AP targets PRDM16 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PR domain containing 16
别名 MEL1, MDS1/EVI1-like gene 1, MDS1/EVI1 like gene 1, KIAA1675, Histone-lysine N-methyltransferase PRDM16
计算分子量 140 kDa
观测分子量~150 kDa
GenBank蛋白编号NM_022114
基因名称 PRDM16
Gene ID (NCBI) 63976
RRIDAB_3086444
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HAZ2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRDM16 antibody 55361-1-APDownload protocol
IHC protocol for PRDM16 antibody 55361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols