验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, Jurkat |
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 2 publications below |
产品信息
55404-1-AP targets PLIN4 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, goat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | KIAA1881 |
| 别名 | KIAA1881, S3-12, S3 12, Perilipin 4, Adipocyte protein S3 12 |
| 计算分子量 | 141 kDa |
| 观测分子量 | 141 kDa |
| GenBank蛋白编号 | NM_001080400 |
| 基因名称 | KIAA1881 |
| Gene ID (NCBI) | 729359 |
| RRID | AB_2881321 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96Q06 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLIN4 (Perilipin 4) is a member of the perilipin family, a group of proteins that coat lipid droplets in adipocytes, the fatty tissue cells responsible for storing fat. The proteins in the Perilipin family are crucial regulators of lipid storage. PLIN4 expression is elevated in obese animals and humans. Plin4 is a lipid droplet protein (LDP) found predominantly in white adipose tissue (PMID: 23423172). It has lower expression in the heart, skeletal muscle, and brown adipose tissue (PMID: 17878492).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLIN4 antibody 55404-1-AP | Download protocol |
| IHC protocol for PLIN4 antibody 55404-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Neurosci TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates. | ||
J Lipid Res Hormone-sensitive lipase protects adipose triglyceride lipase-deficient mice from lethal lipotoxic cardiomyopathy. | ||
Ann Clin Transl Neurol Subsarcolemmal and cytoplasmic p62 positivity and rimmed vacuoles are distinctive for PLIN4-myopathy | ||
Redox Biol Plin4 exacerbates cadmium-decreased testosterone level via inducing ferroptosis in testicular Leydig cells | ||
Biol Reprod NR4A1-mediated regulation of lipid droplets in progesterone synthesis in goat luteal cells† |
