• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Jurkat using 60005-1-Ig (same clone as 60005-1-PBS)

    WB analysis of Jurkat using 60005-1-Ig (same clone as 60005-1-PBS)

    Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 60005-1-Ig (CD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60005-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 60005-1-Ig (same clone as 60005-1-PBS)

    WB analysis of Jurkat using 60005-1-Ig (same clone as 60005-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60005-1-Ig (CD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60005-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60005-1-Ig (same clone as 60005-1-PBS)

    IHC staining of human tonsillitis using 60005-1-Ig (same clone as 60005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60005-1-Ig (CD2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60005-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60005-1-Ig (same clone as 60005-1-PBS)

    IHC staining of human tonsillitis using 60005-1-Ig (same clone as 60005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60005-1-Ig (CD2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60005-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51114-3

    Cytometric bead array standard curve of MP51114-3

    Cytometric bead array standard curve of MP51114-3, CD2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60005-1-PBS. Detection antibody: 60781-4-PBS. Standard:Eg0138. Range: 6.25-100 ng/mL.

CD2 Monoclonal antibody, PBS Only (Capture)

CD2 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 60005-1-PBS

产品说明书

CloneNo. 3A10B2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor, CD2 molecule

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

60005-1-PBS targets CD2 as part of a matched antibody pair:

MP51114-3: 60005-1-PBS capture and 60781-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 CD2 fusion protein Ag0423 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CD2 molecule
别名 LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor, CD2 molecule
计算分子量 351 aa, 39 kDa
观测分子量 50 kDa
GenBank蛋白编号BC033583
基因名称 CD2
Gene ID (NCBI) 914
ENSEMBL Gene IDENSG00000116824
RRIDAB_2881350
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP06729
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.